喉癌患者外周血树突状细胞的体外培养

Generation of Dendritic Cells from Peripheral Blood of Patients with Laryngeal Carcinoma

目的探讨从喉癌患者外周血大量诱导树突状细胞(Dendritic cells,DCs)的有效方法。方法直接从喉癌患者外周血分离DCs;用粒/巨噬细胞集落刺激因子(GM-CSF)、白介素-4(IL-4)刺激喉癌患者外周血贴壁单核细胞(PBMCs);检测培养前后DCs表面HLA-DR、B7-2表达水平及DCs诱导T细胞增殖能力。结果联合应用GM-CSF及IL-4可以从PBMCs中诱导大量高免疫活性的DCs,并通过增加其表面HLA-DR、B7-2表达而增强DCs免疫功能。结论用GM-CSF及IL-4可以从喉癌患者外周血中诱导出大量高免疫活力DCs。

Objective The present study aims at finding a rapid method for inducing dendritic cells （DCs） from Laryngeal Carcinoma patients. Methods DCs were isolated directly from Peripheral Blood of Laryngeal Carcinoma patients; DCs were induced from adherent peripheral blood mononuclear cells （PBMCs） by being cultured with granlocyte/macrophage colony - stimulating factor （ GM - CSF） and interleukin 4 （ IL -4） ; then the expression levels of HLA - DR and 137 -2 on DCs were detected, so was the ability of DCs to induce immune response after and before being cultured with cytokines. Results PBMCs were capable of differentiating into DCs under the stimulation of cytokines which enhanced the aility of DCs to induce Tlymphocyte proliferation by upregulating the expression level of HLA - DR and 137 - 2 on DCs. Conclusions Combination of GM - CSF and IL - 4 can induce the proliferation of DCs with high immune function.