大肠腺癌组织中VEGF表达及微血管形成的临床病理意义

Clinicopathologic Significance of Expression of VEGF and Angiogenesis in Colorectal Adenocarcinoma

目的探讨大肠腺癌组织中血管内皮生长因子(Vascular endothelial growth factor,VEGF)的表达及微血管形成的临床病理意义。方法应用免疫组化S-P法,检测70例大肠腺癌中VEGF蛋白的表达,应用CD34抗体标记微血管并测量微血管密度(Microvessel density,MVD),分析二者与大肠腺癌临床病理特征的关系。结果VEGF蛋白的表达及MVD与不同组织学分化程度无明显相关性(P>0.05);在有淋巴结转移组VEGF的阳性率、MVD值明显高于无淋巴结转移组VEGF的阳性率、MVD值;浸润至浆膜层的VEGF的阳性率及MVD值明显高于浸润至肌层组VEGF的阳性率及MVD值(P<0.01);不同DUKES分期组间相互比较,差异有统计学意义(P<0.05)。结论VEGF的过表达、高MVD值可能对大肠腺癌的侵袭、转移有促进作用。

Objective To study the clinicopathologic significance of expression of vascular endothelial growth factor （VEGF） and angiogenesis in colorectal adenocarcinoma. Methods Immunohistochemical method was used to detect the expression of VEGF and CD34 antibody was used to measure Microvessel density （MVD） , then the clinicopathologic significance was analyzed by 70 specimens of colorectal adenocarcinoma. Results No positive statistical significance was obtained in the correlation among the expression of VEGF, MVD and degree of differentiation （P 〉 0.05） , but higher positive rates of VEGF proteins and higher MVD were found in the group of lymph node metastasis than those of the group without lymph node metastasis, higher positive rates of VEGF proteins and MVD in the group of serous membrane infiltration than those in the group of muscular layer infiltration. Significant difference were statistically observed by positive rates of VEGF proteins and MVD compared in different DUKES stage（ P 〈 0.05, respectively）. Conclusions The over - expression of VEGF and high MVD might promote the invasion and metastasis of colorectal carcinoma.