卡托普利对小鼠在肺部照射后TGF-β_1、TNF-α水平的影响

The effect of captopril on the levels of transforming growth factor β1 and tumor necrosis factor α in mice after lung radiation injury

目的:探讨卡托普利在小鼠放射性肺损伤中对血清转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)的干预影响。方法:雄性C57/BL小鼠,设对照组、右肺单次照射10Gy组、右肺单次照射10Gy+卡托普利组,于照射后36h、7d、15d、30d采集血液标本,ELISA法测血清中TGF-β1及TNF-α;同期取右肺组织以HE染色制成病理学标本。结果:10Gy照射组在照射后不同时段血清中TGF-β1水平及TNF-α水平均较对照组升高;而单次照射10Gy+卡托普利组则表现为在不同时段均较对照组水平降低。析因分析结果为时间因素间、处理因素间、交互作用间均有统计学差异(P<0.05)。病理结果显示10Gy照射组出现进行性加重的炎症改变,而单次照射10Gy+卡托普利组则与对照组比较无明显区别。结论:卡托普利可以减低小鼠肺照射时TGF-β1、TNF-α等细胞因子浓度,并抑制放射性肺损伤的发生。

Objective：To investigate the effect of angiotensin-converting enzyme inhibitors （ACEI）, captopril （Cap）, on the serum levels of transforming growth factor β1 （TGF-β1） and tumor necrosis factor α （TNF-α） in mice after lung irradiation injury. Methods：Male C57/BL mice were divided into three groups： control group, right lung single irradiation group （ 10 Gy）, and right lung single irradiation （ 10 Gy） plus Cap administration group. The blood samples were collected at 36 h, 7 d, 15 d, and 30 d after irradiation. The levels of TGF-β1 and TNF-α were evaluated with ELISA method. The right lungs were isolated and stained with HE for pathological examination at the same time points. Results：The average serum levels of TGF-β1 and TNF-α in single 10-Gy irradiation group were significantly increased at different time points compared with the control group. But they were significantly decreased at different time points in 10-Gy radiation plus Cap group compared with the control group. The results of analysis of variance showed that there was a significant difference among the factor of time, the factor of treatment, and the factor of interaction （ P 〈 0.05 ）. Pathological examination showed that acute inflammation was aggravated in single 10-Gy irradiation group. But there was no significant difference in lung injury score between 10-Gy irradiation plus Cap group and control group. Conclusions： Cap reduces the serum levels of TGF-β1 and TNF-α and prevents lung against radiation-induced injury.