高效液相色谱法测定保健食品中淫羊藿苷

Detection of barrenwort in health food by HPLC

目的:建立高效液相色谱法测定保健食品中淫羊藿苷的方法。方法:样品用30%乙醇提取,经0.22μm滤膜过滤,以甲醇-水为流动相,进入高效液相色谱进行分析。结果:通过实验对最佳波长、柱温、流动相的比例进行选择,获得理想的分离效果,方法最低检出浓度为0.1μg/ml;方法线性范围为0.1μg/ml～0.8 mg/ml;方法平均回收率为101.6%;相对标准偏差<10%。结论:该法简便、快速、定量准确、精密度好,适合于保健食品中淫羊藿苷的测定。

Objective：The high performance liquid chromatography （HPLC） method is employed for determining barrenwort of the health food. Methods：The sample was firstly distilled by using 30% ethanol, filtrated through 0. 22μm membrane, then detected through HPLC with methanol - water as the mobile phase. Results ： The optimum wave - length, the column temperature, the ratio of mobile phase were selected respectively and the good separation effect was achieved. It was shown that the lowest de- tecting concentration was 0. 1μg/ml, the linearity range 0. 1 μg/ml-0. 8 mg/ml, the average recovery 101.6% , and the RSD lower than 10%. Conclusion：The method is easy, fast and precise and can fit for determining barrenwort in the health food.