短尾猴样品中常见哺乳动物及人源污染的特异性研究

Specific detection method to distinguish Tibetan macaca from human and common animal samples

为检测在野外通过非损伤取样法采集到的短尾猴样品,是否受到其它常见哺乳动物及人源的污染,收集包括短尾猴(Macaca thibetana)、人以及7种常见哺乳动物的毛发、血液、粪便、精液或肌肉组织样品,用14对微卫星引物扩增不同物种的STR位点。结果表明,FGA位点在常见动物猪、羊、牛、狗、兔、小白鼠及豚鼠都未见扩增产物:TH01等14对引物在人和短尾猴中均能扩增,扩增产物长度未见明显差异;F13A01产物长度在人和短尾猴之间差异显著。因此,通过FGA、F13A01特异性座位的扩增可以排除短尾猴样品是否受到人及常见哺乳动物的污染。此方法简单、稳定性高、可重复性好,在短尾猴的分子遗传学研究中有较大的应用价值。

To detect whether Tibetan macaca samples were contaminated by human and other common animals＇ biological samples like hairs, blood, faeces, sperm and muscle tissues of Tibetan macaca. Human and seven other kinds of common animal samples were collected for PCR. DNA was quantified after extraction and purification, 14 microsatellite primers were used in this study. The amplification products of Tibetan macaca FGA loci have one band which is almost the same as in human being, no products were found in rabbit, pig, cattle, rat, cavy, sheep and dog; the TH01, CSF10PO,F13A01 and all the other loci can be amplified in human and Tibetan macaca, but the difference was only found in product F13A01. Those results suggested that the primers for human FGA, F13A01 loci could be used to distinguish Tibetan macaca from human beings and common animals. They are two useful and sensitive STR loci in Tibetan macaca＇s molecular genetics study.