天麻生长过程中酸性磷酸酶的研究

Study on acid phosphatase during the development of Gastrodia elata growth

以蜜环菌（Armillaria mellea）伴生的天麻（Gastrodia elata）块茎为材料，研究了天麻生长发育过程中酸性磷酸酶（ACP）活性及其同工酶的变化。结果表明，在天麻生长90d时，其酶谱中有3条谱带，且均显色较弱；生长至120d时，其酶谱与生长90d的酶谱相同，但谱带显色加深；而在生长150d时，酶谱中缺少了一条Rf0．852的谱带。在90—150d的天麻生长发育过程中，酸性磷酸酶（ACP）活性呈现先升高后下降的趋势。讨论了酸性磷酸酶（ACP）同工酶变化的生理学意义。

The change of acid phosphatase （ACP） activity and its isozyme during the development of Gastrodia elata growth together with Armillaria mellea were determined. 3 bands of isozyme were observed on the 90^th day, but the same bands of isozyme zymogram on the 120^th day showed deeper colour. While the band of Rf 0. 852 disappeared on the zymogram of 150^th day. During the development stage from 90d to 150d, activity of ACP increased firstly and decreased finally.