基于二氧化硅微颗粒促细胞增殖效应的基因转染新方法

A New Gene Transfection Based on the Promotion Effect of Silica Microparticles on Cell Proliferation

报道了二氧化硅微颗粒(SiMPs)的促细胞增殖效应,并基于该效应发展了一种以二氧化硅微颗粒为转染伴侣的基因转染新方法,采用MTT实验证明了二氧化硅微颗粒具有促细胞增殖效应,并以绿色荧光蛋白表达载体质粒pEGFP为报告基因,COS-7细胞为靶细胞,在多聚-L-赖氨酸介导的基因转染中,利用二氧化硅微颗粒的促细胞增殖效应,加入二氧化硅微颗粒作为转染伴侣开展基因转染实验,获得了显著增强的基因转染效率,相比于未加入二氧化硅微颗粒为转染伴侣的基因转染方法,该方法的转染效率提高到5倍多.利用二氧化硅微颗粒的促细胞增殖效应,以其作为转染伴侣的基因转染新方法不仅为相关研究提供了一种高效简便的基因转染新方法,而且也为基因转染效率的提高提供了新的思路.

Promotion effect of silica microparticles （SiMP） on cell proliferation was reported. Taking advantage of this effect, a new gene transfection method utilizing SiMP as transfection helpmates was developed. An MTT assay was performed to demonstrate the promotion effect of SiMP on cell proliferation. Based on this effect, SiMP acting as transfection helpmates successfully enhanced poly-L-lysine （PLL） mediated gene transfection. This transfection method showed an above 5-fold increase in transfection efficiency compared with the PLL method that lacked the assistance of SiMP in the delivery of plasmid pEGFP, which encodes green fluorescence protein into COS-7 cells. This new transfection method provides a new strategy to increase transfection efficiency from the angle of promoting the target cell proliferation and viability.