摘要
采用ASE200快速溶剂萃取机提取技术和高效液相色谱法,测定黄姜中薯蓣皂甙元的含量。确定了ASE200提取条件以及HPLC测定条件,以甲醇作为流动相,流速1mL/min,UV检测器,测定波长210nm,采用YWGC18柱(150mm×4.6mmi.d.,10μm)。测定皂甙元的线性范围为0.01～2.93g/L,r=0.9996,样品检出限为0.2mg/L,加标回收率为97.1%～99.8%,RSD=1.1%(n=7)。
The method for determination of diosgenin in dioscorea zingiberensis was established. The dioscorea samples were extracted by ASE200 and determinated by HPLC. The analytical column was YWG Cls （150 mm×4.6 mmi. d. , 10μm）. The mobile phase was methanol with flow rate of 1.0 mL/min. The UV detector wavelength was 210 nm. There was a good linear relationship in the range of 0.01 -2.93 g/L（r =0. 9996）. The sample detection limit was 0.2 mg/L. The sample recoveries were in the range of 97.1% -99.8% and RSD was 1.1% （n =7）.
出处
《化学分析计量》
CAS
2007年第4期18-20,共3页
Chemical Analysis And Meterage
