豆豉纤溶酶分离及其特性的研究

采用硫酸铵分级沉淀、SephadexG-100凝胶过滤层析,从豆豉粗酶液中提取纤溶酶。分离后的纤溶酶样品在SDS-PAGE电泳中有一个活性峰呈单一条带,分子量为35kDa,活性回收率为22.5%,纯化倍数为11.7;另一活性峰呈三条带,需进一步分离。特性研究表明:在pH7.0左右,温度40℃时,酶活力最高;该纤溶酶不耐热,其纤溶活性可被Mn2+、Mg2+以及Na2SO3激活,Ca2+以及乙醇、明胶对酶活力具有较强的抑制作用;NaCl浓度低于10%,酶活力能够基本保持稳定。

Fibrinolytic enzyme was separated by （NH4）2O4 fractional precipitation and SephadexG-100 gel filtration from crude enzyme liquid of Douchi. The absorption peak 2 give a single band on SDS- PAGE, its molecular mass is 35 kDa, recovery rate of activity is 22,5% and purify multiple is 11.7. Another active peak has three bands and need to be separated continued. In study of characterization of Douchi fibrinolytic enzyme, it was found that the highest fibrinolytic activity is pH 7.0 and 40℃,the heat stability is not well; the fibrinolytic activity is activated by Mn^2＋.Mg^2＋ and Na2SO3 while inhibited by Ca^2＋、alcohol and gelatin, When concentration of NaCl is lower than 10%, the fibrinolytic activity can keep stability.