大孔吸附树脂法纯化苦瓜皂苷工艺的研究

研究了大孔吸附树脂纯化苦瓜皂苷的方法。确定纯化的最佳工艺条件为:选用AB-8型大孔吸附树脂,吸附液pH为8～9,吸附流速为1.0mL/min,吸附液用量与树脂体积比为8∶1,吸附时间为60min,洗脱剂为70%的乙醇,洗脱剂用量与树脂体积比为8∶1,解吸时间为40min。收集70%乙醇洗脱液,洗脱液浓缩并真空干燥,纯化后可将粗提物皂苷含量提高到69.04%。

The purification of saponins in Bitter Melon by macroporous adsorptive resins was investigated. The optimum purification condition were confirmed as follows： the type of macro-porous resins AB-8, the pH value of absorbing solution 8-9, the absorbing velocity of flow 1.0mL/min, the absorbing time was 60minutes, the concentration of ethanol as elutent was 70%, the proportion of the amount of absorbing solution and resins was 8：1, the proportion of the amount of elutent and resins was 8：1, the eluting time 40 minutes. Elutent solvents were concentrated and vacuum dried. The content of the crude saponins rises to 69.04%.