双水相体系萃取分离L-组氨酸的研究

采用PEG-(NH4)2SO4双水相体系萃取分离L-组氨酸。实验考察了pH、温度、聚乙二醇加入量、L-组氨酸初始浓度、硫酸钠及L-赖氨酸的存在对萃取分离的影响。结果表明:L-组氨酸在该双水相体系的分配系数K随体系pH和聚乙二醇加入量的增大而减小,随着L-组氨酸初始浓度和L-赖氨酸加入量的增大而增大,而温度和Na2SO4的影响不明显;L-组氨酸在该双水相体系的萃取率η随体系pH和聚乙二醇加入量的增大而增大,随着L-组氨酸和L-赖氨酸加入量的增大而减小,而温度和Na2SO4的影响同样不明显。

L-histidine extraction and separation by aqueous two-phase system （PEG-（NH4）2SO4） were studied. Effects of pH value, temperature,quantity of PEG and L-histidine, Na2SO4 and L-lysine on extraction were investigated. The results indicated that the partition coefficient K of L-histidine decreased with the increasing of pH value and quantity of PEG, increased with the increasing of quantity of L- histidine and L-lysine,but the influence was not obvious by the addition of Na2SO4 and temperature. The rate of extraction of L-histidine increased with the increasing of pH value and quantity of PEG, decreased with the increasing of quantity of L- histidine and L-lysine,and the influence was not obvious by the addition of Na2SO4 and temperature.