摘要
采用PEG-(NH4)2SO4双水相体系萃取分离L-组氨酸。实验考察了pH、温度、聚乙二醇加入量、L-组氨酸初始浓度、硫酸钠及L-赖氨酸的存在对萃取分离的影响。结果表明:L-组氨酸在该双水相体系的分配系数K随体系pH和聚乙二醇加入量的增大而减小,随着L-组氨酸初始浓度和L-赖氨酸加入量的增大而增大,而温度和Na2SO4的影响不明显;L-组氨酸在该双水相体系的萃取率η随体系pH和聚乙二醇加入量的增大而增大,随着L-组氨酸和L-赖氨酸加入量的增大而减小,而温度和Na2SO4的影响同样不明显。
L-histidine extraction and separation by aqueous two-phase system (PEG-(NH4)2SO4) were studied. Effects of pH value, temperature,quantity of PEG and L-histidine, Na2SO4 and L-lysine on extraction were investigated. The results indicated that the partition coefficient K of L-histidine decreased with the increasing of pH value and quantity of PEG, increased with the increasing of quantity of L- histidine and L-lysine,but the influence was not obvious by the addition of Na2SO4 and temperature. The rate of extraction of L-histidine increased with the increasing of pH value and quantity of PEG, decreased with the increasing of quantity of L- histidine and L-lysine,and the influence was not obvious by the addition of Na2SO4 and temperature.
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第7期165-167,149,共4页
Science and Technology of Food Industry
基金
福建省科技计划重点项目(2004H013)
泉州市科技计划重点项目(2005G6)
关键词
双水相萃取
L-组氨酸
聚乙二醇
萃取率
分配系数
aqueous two-phase extraction
L-histidine
PEG
rate of extraction
partition coefficient