荧光法测定婴儿奶粉中色氨酸的含量

建立了婴儿奶粉中色氨酸的荧光分析方法,以NaOH为水解液水解婴儿奶粉,在激发波长225nm,发射波长350nm处测定色氨酸的荧光强度。方法线性范围0～0.07mg/L,检测限为0.0015mg/L,回收率在95%～105%之间。此法快速、简便、灵敏度高。

This paper setup a procedure of fluorescence method to measure tryptophan in infant powder. NaOH containing 0.5% soluble starch was used as hydrolytic solution. The fluorescence intensity of tryptophan was determined with excitation wavelength 225nm and emission wavelength 350nm,The linear range of calibration graph was 0- 0.07mg/L.The detection limit was 0.0015mg/L.There covery rate of sample was among 95%-105%.