激光拉曼光谱内标法测定葡萄糖液浓度

Quantitative Determination of Glucose by Internal Standard Laser Raman Spectra

利用葡萄糖溶液的激光拉曼光谱中的特征峰-CCO（1 125 cm^-1）,以本底溶液水为内标物,组成相对强度,对葡萄糖溶液含量进行了测定研究,其线性范围为0~1.8 mol.L^-1,检出限为0.022 7 mol.L^-1,共存的KCl和NaCl不引起干扰,用于葡萄糖氯化钠注射液的测定获得了成功。经测定某市售标识量为葡萄糖氯化钠注射液、5%葡萄糖和10%葡萄糖注射液实测平均浓度分别为4.91%,4.93%和9.92%,其回收率分别在71.88%~126.31%,81.02%~124.89%和74.87%~121.32%之间,RSD为5.44%,4.34%和0.94%。该方法具有测定简单、操作方便,无需添加其他任何化学试剂,是一种绿色分析测定方法。

In the present paper we report quantitative analysis of glucose using internal standard laser Raman spectra. A good linear correlation was observed between the intensities of the --COO band at 1 125 cm^-1 using excition wavelength of 632.81 nm （r=0. 998 8） and the glucose concentration over the range 0-1.8 mol · L^-1. Band intensities were normalized against an internal standard （water band at 1 643 cm^-1）, and the limit of detection （L. O. D. ） of glucose is 0. 022 7 mol · L^-1. The interference ions would not influence the quantitative analysis. When this method was used to determine 5% glucose NaC1, 5% glucose, and 10 % glucose injections, the result showed that the recoveries are 71.88 %-126. 31%, 81.02 %-124.89 % and 74. 87 %-121.32 %, and the RSDs are 5. 44%, 4. 34% and 0. 94%, respectively. The non destructive, non intrusive nature of the method makes internal standard laser Raman spectra a convenient, accurate, and green quantitative analysis method.