毛白杨嵌合体扦插生根相关生理学特性研究

Study on the Physiological Characteristics Related to Cutting Rootage of Chimera in Populus Tomentosa Carr.

为了阐明嵌合体扦插生根和普通毛白杨与过氧化物酶、吲哚乙酸氧化酶、超氧化物歧化酶和可溶性蛋白含量的关系。对在河北省宁晋县获得的毛白杨嵌合体的生根机制进行了动态跟踪分析,测定了过氧化物酶（POD）、吲哚乙酸氧化酶（IAAO）及超氧化物歧化酶（SOD）的活性和可溶性蛋白含量。嵌合体POD活性在第9天升至高峰,第18天降至低谷,第21天又出现高峰,而毛白杨在第12天出现高峰,第24天出现低谷;IAAO活性变化规律在前期与POD相似;嵌合体SOD活性开始呈下降趋势,在第6-9天出现低谷,之后有所升高但无太大变化,而毛白杨开始呈上升趋势,第9天形成高峰,之后有所下降,但规律不明显;可溶性蛋白含量嵌合体总体比毛白杨高,且两者在第15天变化相反。嵌合体易生根与其PODI、AAO及SOD活性和可溶性蛋白含量密切相关,是四者协同作用的结果。

The aim of the study was to clarify the relationships between the cutting rootage of the chimera and the normal Populus tomentosa Carr.and the activities of peroxidase（POD）,indole acetic acid oxidase（IAAO） and super oxide dismutase（SOD） and the content of dissoluble protein.Based on dynamic trace analysis of the rooting mechanism of chimera in P.tomentosa from Ningjin county of Hebei province,the activities of POD,IAAO and SOD,and soluble protein content were determined.The POD activity in chimera reached its first peak on the 9th day,followed by a valley on the 18th day,and then the second peak on the 21st day.While the POD activity in P. tomentosa reached its peak on the 12th day,and then appeared a valley on the 24th day. The change law of IAAO activity was similar to that of POD activity in growth prophase. The SOD activity in chimera showed a trend of reducing at the beglnning,appeared a valley during the 6th - 9th day,and then increased without much change. While the SOD activity in P. tomentosa showed a trend of fast increasing at the beginning,appeared a peak on the 9th day, and then reduced,but its change law was not obvious. The soluble protein content in chlmera was higher than that in P. tomentosa ,and their changes were reverse on the 15th day. Chimera rooted easily,which was closely related to activities of POD,L4AO and SOD,and soluble protein content,was the result of the synergy of these four.