F_1大丽花组培苗快繁技术研究

Study on the Technology of Tissue Culture and Rapid Reproducing of Dahlia pinnata F_1

用F1大丽花的种子、嫩芽及开花茎段做外植体,以MS为基本培养基,在5个分化培养基上接种诱导出芽,在4个继代培养基上扩繁增殖,在4个生根培养基上诱导生根,在4种无土基质上出瓶培养成生产用苗。得出F1大丽花组培苗速繁适用程序为:嫩芽及开花茎段为外植体→接种在MS+6-BA 0.5 mg/L+NAA 0.2 mg/L→增殖在MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+B90.1mg/L→生根在1/2MS+0.1 NAA mg/L→落叶松针叶+细沙出瓶培养。试验采用简易材料和药剂,降低了组培苗生产成本,选取可控花期的不同部位外植体,可缩短育苗周期。

The seed,bud and stem of Dahlia pinnata F1 were selected as explants, MS was used as the fundamental culture medium including 5 kinds of different media,4 kinds of generation reproduce media,4 kinds of rooting media,and 4 kinds of ground substances for plantlets-transplating.The preliminary conclusion was： the suitable inducing medium for bud and stem was MS ＋ 6-BA 0.5 mg/L＋NAA 0.2 mg/L,the generation medium was MS ＋ 6-BA 1.0 mg/L＋ NAA 0.2 mg/L＋ B9 0.1 mg/L,the rooting medium was 1/2MS ＋ NAA 0.1 mg/L and the suitable ground substance was Pine needle ＋ sand. In this experiment the simple material and the medicament were used, which reduced the plantlet production cost, and the selection oi controllable flowering season in different position explants was allowed to reduce the cycle of plantlet regeneration.