鼻咽癌病人血清抗重组EB病毒Zta抗原IgG抗体检测

ANTIBODY RESPONSE TO RECOMBINANT EPSTEIN-BARR VIRUS Zta PROTEIN IN PATIENTS WITH NASOPHARYNGEAL CARCINOMA

目的:用免疫印迹法检测鼻咽癌病人血清中抗EB病毒Zta-IgG抗体。方法:利用含EB病毒BZLF1基因完整开放读框的pGEX2TK-ZcDNA质粒转染大肠杆菌,诱导产生并提纯重组基因产物GST-Zta,以GST-Zta作为抗原,用免疫印迹法检测24例鼻咽癌病人血清中Zta-IgG抗体,28例正常人血清作为对照。结果:鼻咽癌组血清中91.7%Zta-IgG阳性,对照组中10.7%Zta-IgG阳性,两组差异有统计学意义(P=0.000)。结论:免疫印迹法检测鼻咽癌病人血清中Zta-IgG抗体有较高的敏感性和特异性,可能可以作为鼻咽癌病人诊断筛选的指标。

Objective： This study aims to investigate IgG antibody response to Epstein Barr virus Zta protein in patients with nasopharyngeal carcinoma（NPC） by a western bolt method. Methods： glutathione-S-transferase （GST） recombinant Zta was prepared and was used as antigen in western blot. Serum samples derived from 24 patients with NPC, 28 healthy volunteers were examined for the presence of antibodies directed against Zta. Result： Among the patients with NPC, 91.7% showed a positive IgG response to Zta, In contrast, the reactions were positive in only 10.7% of healthy volunteers. Conclusion： The western blot method described for the detection of IgG antibodies directed against recombinant Zta protein is simple and may be useful as a serologic parameter for the screening and diagnosis of patients with NPC.