中药八角茴香的毛细管电泳指纹图谱以及莽草酸的含量测定

Capillary electrophoresis fingerprint for Illicium verum Hook.f.and determination of shikimic acid in its extract

目的:获得八角茴香的毛细管电泳指纹图谱并对其中莽草酸的含量进行测定。方法:选用广西八角茴香为道地药材,莽草酸为对照物,采用毛细管电泳法(运行缓冲液:5mmol·L^(-1)四硼酸钠,60mmol·L^(-1)SDS;运行电压:12.5kV;电进样时间:3s;检测波长:213nm)分析了7个不同产地八角茴香提取物的成分及含量差异,特别是莽草酸含量差异,并利用模糊相似优先比算法评价了不同产地八角茴香与道地八角茴香的相似度。结果:获得了八角茴香的指纹图谱,检测到的9个相对位置稳定的共有峰可作为鉴别八角茴香的指标峰;测定了不同产地八角茴香中莽草酸的含量。结论:本研究获得的八角茴香的毛细管电泳指纹图谱有助于进行八角茴香的真伪鉴别和质量控制;对莽草酸含量的测定,可用以指导各地八角茴香的充分利用。

Objective： To get the capillary electrophoresis （CE） fingerprint of Illicium verum Hook. f. and determine the content of shikimic acid in them from different habitat. Method： Taking an Illicium verum Hook. f. from Guangxi province as the genuine traditional Chinese drugs and shikimic acid as a reference component, the differences of constituents and their content, especially the content of shikimic acid were analyzed by CE （The running buffer was 5mmol·L^-1 Na2B4O7 · 10H2O,60 mmol · L^-1 SDS;the separation voltage was 12. 5 kV;the sample was injected into the capillary by electrokinetics in 3 s;the detection wavelength was 213 nm） ,and Illicium verum Hook. f. from seven different provinces were compared with Guangxi＇s using priority ratio of fuzzy equiform. Results： The CE fingerprint of Illicium verum Hook. f. was established. Nine common peaks correlated position were obtained and used to definite the index peak of Illicium verum Hook. f. ; in addition, the content of shikimic acid was determined. Conclusion： CE fingerprint of Illicium verum Hook. f. here can be expected to identify the genuine or sham of Illicium verum Hook. f. on the quality control for this traditional Chinese medicine;the assay of shikimic acid can guide the full use of different Illicium verum Hook. f..