HPLC法测定银丝保片中葛根素含量

HPLC determination of puerarin in Yinsibao Tablets

目的:比较不同提取方法对高效液相色谱法测定葛根素含量的影响。方法:采用 Shim pack CLC-ODS 色谱柱(4.6mm×250mm,5μm),以0.1%磷酸水溶液-甲醇(75:25)为流动相,流速1.0mL·min^(-1),检测波长249nm,柱温40℃。结果:葛根素在12.05～192.8μg·mL^(-1)时,浓度与峰面积呈良好的线性关系 Y=6.7825×10~4X-1.21071×10~5(r=0.9995),最低检出限8.19ng·mL^(-1),加标回收率98.85%,相对标准偏差(RSD)1.82%。结论:该方法操作简便,结果准确。

Objective： A HPLC analysis method was established for the determination of Puerarin and comparison of different extracting methods. Methods：Separation and determination under following conditions：Shim pack CLC - ODS （4.6mm×250mm,5μm） column, a mobile phase consisting of 0. 1% phosphoric acid ： methanol （ 75 ： 25 ）, detection length of UV at 249 nm, flow rate of 1.0 mL·min^-1, column temperature of 40 ℃. Results： There is good linear relationship within the range of 12. 05 - 192. 8 μg·mL^-1 of Puerarin, Y = 6. 7825×10^4X - 1. 21071× 10^5 （ r = 0. 9995 ）. The detecting limit is 8. 19 ng·mL^-1, and recovery rate of 98. 85 %, with a RSD of 1.82%. Conclusion：The HPLC methods are simple and accurate.