毛细管气相色谱法测定无极膏中薄荷脑、樟脑、水杨酸甲酯、冰片及麝香草酚的含量

GC determination of mentholum,camphora,borneolum syntheticum,menthylsalicylate and thymol in Wuji cream

目的:建立 GC 法测定无极膏中薄荷脑、樟脑、水杨酸甲酯、冰片及麝香草酚含量的方法。方法:采用 HP-5弹性石英毛细管柱程序升温的方法测定。结果:5组分 GC 法测定的分离度和线性关系良好,薄荷脑、樟脑、水杨酸甲酯、冰片、麝香草酚线性范围分别为132.4～1324.0μg·mL^(-1),243.9-2439.0μg·mL^(-1),132.0～1320.0μg·mL^(-1),20.0～200.0μg·mL^(-1),10.1～101.3μg·mL^(-1);平均回收率分别为99.8%(RSD=0.9%),99.5%(RSD=1.2%),98.7%(RSD=0.9%),99.5%(RSD=1.2%),100.8%(RSD=1.6%)。结论:本法准确、灵敏,可用于该制剂的质量控制。

Objective：To establish a method for determination of mentholum, camphora, borneolum syntheticum, menthylsalicylate and thymol in Wuji cream. Method：The determination was carried out on a HP -5 capillary column with sequential increase of temperature programming. Results： The linear ranges of mentholum, eamphora, borneolum syntheticum, menthylsalicylate and thymol were 132.4 - 1324. 0 μg·mL^-1 , 243.9 - 2439.0 μg·mL^-1, 132. 0 - 1320.0 μg·mL^-1,20. 0 - 200. 0 μg·mL^-1 and 10. 1 - 101.3μg·mL^-1, respectively ; the average recoveries were 99.8% （ RSD = 0. 9% ）, 99.5% （ RSD = 1.2% ）, 98.7% （ RSD = 0. 9% ）, 99. 5% （ RSD = 1.2% ）, 100. 8% （RSD = 1.6% ）. Conclusion：The method is simple, sensitive and accurate. It could be used for quality control of Wuji cream.