HPLC法测定三号金龙片中士的宁的含量

HPLC determination of strychnine content in Sanhao Jinlong tablets

目的:采用高效液相色谱法测定三号金龙片中士的宁的含量。方法:采用 Thermo-C_(18)色谱柱(250mm×4.6mm,5μm),以乙腈-0.01mol·L^(-1)庚烷磺酸钠与0.02mol·L^(-1)磷酸二氢钾等量混合溶液(用10%磷酸调节 pH 2.8)(21:79)为流动相,流速为1.0 mL·min^(-1),检测波长为260nm,柱温为室温。结果:士的宁进样浓度在10.5～70μg·mL^(-1)的范围内与峰面积呈良好的线性关系(r=0.9998),回收率(n=5)为98.9%。结论:本方法操作简便,精密度好,结果准确可靠。

Objective：To determine strychnine content in Sanhao Jinlong tablets by HPLC. Methods：Thermo -C18 column（250 mm ×4.6 mm,5 μm） was used. Acetonitrile -0.01 mol·L^-1 sodium heptanesulfonate and 0. 02mol·L^-1 potassium dihydrogen phosphate equivalent mixture solution（ 10% phosphoric acid was used to regulate pH 2.8） （21： 79） as the mobile phase. The flow rate was 1.0 mL·min^-1 ,the detected wavelength was 260 nm,the temperature of column was controlled at room temperature, Results：The calibration curve was linear in the range of 10. 5 -70 μg·min^-1 for strychnine （ r = 0. 9998 ）. The average recovery （ n = 5 ） was 98.9%. Conclusion： The method is simple, accurate and reliable.