乙醇诱导大鼠肝脏超微结构改变与护肝灵的干预

The ultrastructure changes of rat liver induced by alcohol and the intervention with Huganling capsules

目的观察护肝灵对乙醇诱导的大鼠肝脏超微结构改变的影响。方法以乙醇灌胃法诱导大鼠肝损伤模型,护肝灵高、低剂量干预,易善复为阳性对照,连续42 d,H-600 IV型透视电镜观察肝细胞超微结构和糖原。结果乙醇灌胃各组大鼠的肝细胞出现不同程度的核固缩、线粒体肿胀、内质网扩张,偶见酒精小体(Mallory小体);细胞内出现脂滴;细胞间出现胶原纤维;糖原减少或消失。护肝灵低剂量组与阳性药相当,肝细胞核轻微固缩;线粒体肿胀明显,嵴断裂消失,粗面内质网扩张;偶见糖原。高剂量组肝细胞超微结构清晰,优于阳性药,与阴性对照组比较无显著差异。结论护肝灵对乙醇诱导的大鼠肝细胞超微结构破坏有保护作用。

OBJECTIVE To explore the effect of Huganing capsules on uhrastructure changes of rat liver induced by alcohol. METHODS Liver injury model of the rat was induced by ig alcohol for 42 days and meantime Huganling capsules was administrated. Yishanfu capsules was used as positive control. The uhrastructure and glycogen of rat liver hepatic cell were observed by transmission electron microscope（ H -600Ⅳ ）. RESULTS Hepatic cells in all the groups administrated with alcohol exhibited karyopycnosis, mitochondrion engorgement, endoplasmic reticulum extension and rare Mallory body. Lipid droplet was seen in the cell, as well as collagen fiber was seen between cells. Glycogen decreased or even disappeared. In low dose of Huganling capsules group, there was slightly karyopycnosis, mitochondrion engorgement and the ridge of mitochondrion broken or disappeared and rough endoplasmic reticulum extended,with rare glycogen. So there was no difference compared with positive group. In high dose of Huganling capsules group, ultrastructure of the rat hepatic cells was clear, whose effect was better than that of positive one, epual to that of negative group. CONCLUSION Huganling capsules could prevent rat hepatic cell uhrastructure from alcohol＇ s destruction.