摘要
目的研究注射用血塞通、血栓通注射液及注射用血脉通的化学成分,为注射用血脉通立项提供实验依椐。方法用symetry shield C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水二元梯度洗脱;流速1.0 ml.min-1;分别以ELSD和UVD为检测器,ELSD测定参数为漂移管温度115℃;气体(氮气)流量1.5 L.min-1,检测波长210 nm。在此色谱条件下,将3种药品的主要组分分别展开,表达成色谱指纹图谱,并对主组分进行结构识别,归属跟踪,对各自的有效成分进行了分析和比较。结果注射用血塞通、血栓通注射液均为三七的总皂苷,有效成分基本相似,仅存在组成比例波动,其主要差别是剂型不同;注射用血脉通的主组分为三七三醇皂苷,与前两制剂的物质基础存在较大差异。结论通过指纹图谱可以对三七及其制剂的有效成分进行测定。
OBJECTIVE To analyze the effecient elements of Xuesaitong for injection, Xueshuantong injection and Xuemaitong for injection by fingerprint. METHODS A primary analysis was carried out on symetry shield C18 column (250 mm×4.6 mm,5 μm) , with acetonitrile and water as mobil phase ( griadient flow ) and flow rate of 1.0 ml· min^ - 1. ELSD and UVD were used as detectors. The temperature of drift tube was 115℃ with N2 flow rate of was 1.5 L·min^-1 for ELSD, while detection wavelength was 210 nm for UVD. CONCLUSION The difference lies in the effecient elements of Xuesaitong for injection, Xueshuantong injection and Xuemaitong for injection. RESULTS The fingerprint is suitable for the determination of notoginseng and its injections.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第1期76-78,共3页
West China Journal of Pharmaceutical Sciences