摘要
目的 测定脉络宁注射液及玄参中肉桂酸的含量。方法以甲醇-水-冰乙酸(45:55:0.2)为流动相,采用C18化学键合硅胶为固定相,在273nm测定。结果 肉桂酸在0.027~0.163μg范围内线性关系和精密度良好,平均回收率为97.8%。结论方法简便、可靠、准确,可用于该制剂的质量控制。
OBJECTIVE To establish an HPLC method for determination of cinnamic acid in Mailuoning injection and Scrophularia ningpoensis Hemsl.. METHODS The method used a C18 column as analysis column and methanol- H2O- acetic acid (45:55:0.2) as the mobile phase at a flow rate of 1.0 ml·min^-1 with detection wavelength at 273 nm. RESULTS The standard curve was linear within the concentration range of 0. 027 -0. 163 μg. The average recovery was 97.8%. CONCLUSION The method is simple, reliable, accurate and can be used to the quality control of the preparation.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第1期91-92,共2页
West China Journal of Pharmaceutical Sciences
关键词
HPLC
脉络宁注射液
玄参
肉桂酸
含量测定
HPLC
Mailuoning injection
Scrophularia ningpoensis Hemsl.
Cinnamic acid
Assay