HPLC测定脉络宁注射液及玄参药材中肉桂酸的含量

Determination of cinnamic acid in Mailuoning injection and Scrophularia ningpoensis Hemsl. by HPLC

目的 测定脉络宁注射液及玄参中肉桂酸的含量。方法以甲醇-水-冰乙酸（45：55：0．2）为流动相，采用C18化学键合硅胶为固定相，在273nm测定。结果 肉桂酸在0．027～0．163μg范围内线性关系和精密度良好，平均回收率为97．8％。结论方法简便、可靠、准确，可用于该制剂的质量控制。

OBJECTIVE To establish an HPLC method for determination of cinnamic acid in Mailuoning injection and Scrophularia ningpoensis Hemsl.. METHODS The method used a C18 column as analysis column and methanol- H2O- acetic acid （45：55：0.2） as the mobile phase at a flow rate of 1.0 ml·min^-1 with detection wavelength at 273 nm. RESULTS The standard curve was linear within the concentration range of 0. 027 -0. 163 μg. The average recovery was 97.8%. CONCLUSION The method is simple, reliable, accurate and can be used to the quality control of the preparation.