川黄颗粒的薄层鉴别及丹参素钠的含量测定

Identification and content determination of Chuanhuang granule

目的 建立川黄颗粒鉴别和含量测定的方法。方法 采用TLC法和HPLC法。结果 TLC鉴别川黄颗粒中的何首乌、黄芪、党参、枸杞子等药材；HPLC测定丹参素钠的含量，丹参素钠在0．26～1．84mg的范围内，线性关系良好（r=0．9996）。平均回收率为99．8％，RSD=1．4％。结论 鉴别重复性好、专属性强，含量测定方法简便、准确。

OBJECTIVE To establish indentification and content determination method of Chuanhuang granules. METHODS TLC and HPLC were used. RESULTS TLC methods could indentify Radix Polygoni Multiflori, Radix Astragali, Radix Codonopsis and Fructus Lycii. HPLC method could quantitatively determine sodium danshensu content in Chuanhuang granules. The linear range of sodium danshensu was 0.26 - 1.84 rag（ r = 0.9996）. The average recovery was 99.8% with RSD of 1.4%. CONCLUSION Indentification method is reproducible and specific, quantitative determination method is simple and accurate.