HPLC测定清热达郁口服液中梓醇的含量

Determination of catalpol in Qingre Dayu oral solution by HPLC

目的 建立测定清热达郁口服液中梓醇含量的方法。方法 采用HPLC法，测定清热达郁口服液中的梓醇含量，Dia—monsilC18（250mm×4．6mm，5μm）柱，流动相乙腈-0．1％磷酸溶液（1：99），流速1．0ml·min^-1，检测波长210nm。结果梓醇在1．1～8．8μg范围内线性关系良好（r=0．9999），其平均回收率为98．80％（RSD=1．2％，n=5）。结论 所建方法简便、准确、可靠，可用于清热达郁口服液的质量控制。

OBJECTIVE To establish an HPLC method for determing catalpol in Qingre Dayu oral solution. METHODS The determination was carried out with Diamonsil C18 column（250 mm ×4.6 mm,5μm）with acetonitrile -0.1% phosphoric acid （1：99） as the mobile phase ; the detective wavelength was set at 210 nm ; the flow rate was 1.0 ml· min^- 1. RESULTS The calibration curve was linear over the range of 1.1 -8.8μg（r =0. 9999）, The average recovery of the method was 98.80% （RSD = 1.2% ,n =5）. CONCLUSION The results show that this method is rapid, simple, accurate and reliable. It can be used for quality control of Qingre Dayu oral solution.