食管癌患者血清中NGAL蛋白含量检测及其临床意义

Serum NGAL protein concentration of esophageal carcinoma patients and its clinical signification

目的:探讨食管癌患者血清中NGAL蛋白的含量及其临床意义。方法:双抗体夹心ELISA法检测97例食管癌患者和85名正常人血清中NGAL蛋白的含量。联合Western blot和免疫荧光技术检测5种食管癌细胞系表达NGAL蛋白的异质性情况。结果:97例食管癌患者血清中NGAL蛋白含量为(5.94±2.86)μg/L,85名正常人为(4.94±2.58)μg/L,差异有统计学意义,P=0.015。实验数据散点分布结果表明,血清NGAL蛋白含量最高值亚群属于食管癌患者,最低值亚群属于正常人;而多数数据相互交叉。Western blot和免疫荧光检测结果一致,表明SHEEC、EC18和EC8712与EC109和EC171相比,前3种食管癌细胞系表达NGAL蛋白的水平明显高,提示食管癌细胞表达NGAL蛋白异质性明显。结论:食管癌患者血清中NGAL蛋白的含量明显高于正常人。因为异质性所致,通过检测血清中NGAL蛋白含量来判定食管癌的发生发展,其临床意义是有限的。

OBJECTIVE： To investigate the neutrophil gelatinaseassociated lipocalin （NGAL） protein concentration in the sera of patients with esophageal squamous cell carcinoma （ESCC） and its clinical significations. METHODS： A double antibody sandwich ELISA was established to detect the NGAL protein concentration in the serum samples from 97 cases of the ESCC patients and 85 cases of healthy controls. The heterogeneity of NGAL protein expression in the five esophageal carcinoma cell lines was detected by both Western blotting and immunofluorescence technique. RESULTS： The NGAL protein concentration in the 97 cases of ESCC patients was （5.94±2.86）μg/L, while that in 85 cases of healthy controls was （4.94±2.58）μg/L. There was a statistical significance between the two data with P=0. 015. The spot distribution of NGAL protein concentration showed that there was a sub-group of the higher levels in the ESCC patients and a sub-group of lower level in the healthy controls, and most of the date were overlap each other. The NGAL protein levels in SHEEC, EC18 and EC8712 cells were obviously higher than those in EC109 and EC171 cells by using both Western blot and immunofluorescence analysis, which might indicated that there was the heterogeneity of NGAL protein expression in the esophageal carcinoma cells. CONCLUSION： The serum NGAL protein concentration from patients with ESCC is obviously higher than that from healthy controls, but the clinical significations are limited by detecting the serum NGAL protein level to estimate the developmental progression of the esophageal carcinoma, because of the heterogeneity of NGAL orotein expression.