儿茶酚胺代谢限速酶基因多态性与子痫前期关系的研究

The study on the association between the gene polymorphisms of catecholamine metabolism rate-limiting enzyms and preeclampsia

目的:探讨酪氨酸羟化酶(TH)、多巴胺β羟化酶(DβH)、儿茶酚-O-甲基转移酶(COMT)基因多态性与子痫前期的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,分别检测77例子痫前期患者(子痫前期组)及80例年龄及孕周皆相匹配的正常晚期妊娠妇女(对照组)TH基因2606G/A、DβH基因589G/A、COMT基因1947G/A多态性位点频率。结果:①TH、DβH、COMT基因型GG(野生型)、GA(突变杂合子)、AA(突变纯合子)的基因型频率和G、A等位基因频率在子痫前期组与对照组之间的分布差异无显著性(P>0.05)。②TH、DβH、COMT基因型频率在子痫前期组轻、重度孕妇间的分布差异无显著性(P>0.05)。结论:TH基因2606G/A、DβH基因589G/A、COMT基因1947G/A突变多态性与子痫前期的发病及病情轻重无相关性。

Objective： Tyrosine hydroxylase （TH） , dopamine - β - hydmxylase （DβH） and catechol - β - methyltransferase （COMT） are rate - limiting enzymes and play important roles in catecholamine metabolism. The study was to investigate the impacts of these gene polymorphisms on preeclampsia susceptibility. Methods： 77 patients with preeclampsia （preeclampsia group） and 80 normal pregnancies （ control group） matched for age and week of gestation were recruited. Genotyping was performed by using PCR - based restriction fragment length polymorphism （RFLP） analysis. Results： Significant differences were not observed between the preeclampsia group and the control group in the frequencies of TH, DβH , COMT alleles and genotypes. The genotype frequencies of mild, severe preeclampsia groups and control group are not significantly different. Conclusion： The gene polymorphisms of TH 2606G/A, DβH 589G/A, COMT 1947G/A are not associated with the generation of preeclampsia and the severity of preeclampsia.