4个微卫星标记在波尔山羊中的遗传多态性研究

Studies on genetic polymorphism at four microsatellite loci in Bore goats

根据比较基因组学方法,利用绵羊6号染色体上与主效多胎基因(FecB)紧密连锁的4个微卫星标记OarAE101、OarHH55、BM1329和BMS2508,对波尔山羊进行了等位基因频率、多态信息含量、有效等位基因数和杂合度的遗传检测。结果表明,4个微卫星位点在波尔山羊上多态性丰富;OarAE101基因座的多态信息含量(PIC)、有效等位基因数(Ne)、平均杂合度(He)分别为0.847 4,6.553 1和0.862 8;OarHH55基因座分别为0.767 6,4.302 9和0.796 8;BM1329基因座分别为0.841 4,6.305 2和0.857 6;BMS2508基因座分别为0.783 1,4.6104和0.8096。4个微卫星位点均为高度多态位点,可用于波尔山羊遗传多态性评估和多胎性状研究。

According to comparative genomics, the four microsatellite loci including OarAE101, OarHH55,BM1329 and BMS2508 that were linked to the fecundity gene Fec^B in the sheep chromosome 6 were selected to obtain molecular markers in goat. The frequency of allele, polymorphism information content （PIC）,number of effective alleles （Ne） and heterozygosity （He） of corresponding microsatellite locis in Boer goat were detected. The results indicated that there were genetic polymorphisms at these four micro-satellite loci. PIC,Ne,and He at OarAE101 was 0. 847 4,6. 553 1,0. 862 8,respectively;PIC,Ne,and He at OarHH55 was 0. 767 6,4. 302 9,0. 796 8,respectively;PIC,Ne, He at BM1329 was 0. 841 4,6. 305 2, 0. 857 6,respectively;the PIC, Ne, He at BMS2508 was 0. 783 1,4. 610 4,0. 809 6, respectively in Boer goats. The four microsatellite locl were height polymorphic loci which could be used for genetic polymorphism evaluation and study of the fecundity trait in Boer goats.