摘要
用单抗２Ｂ４包被ＥＬＩＳＡ微孔反应板，将处理后的粪样和酶标兔抗ＬａＲＶＩｇＧ同时加入包被孔内孵育，置微型振荡器上振摇，建立了检测粪样中兔轮状病毒（ＬａＲＶ）抗原的单抗－ＥＬＩＳＡ一步法快速诊断技术，与常规ＥＬＳＩＡ、病毒分离培养、电镜、荧光抗体等作了实验室和临床应用比较，结果证明单抗－ＥＬＩＳＡ一步法敏感、特异、快速、简便。
Rotavirus(LaRV)in rabbits was detected by one step method with double antibody sandwich ELISA. Thesamplesandenzyme labelledantibody wereincubatedwith PEG6000 simultaneously,then shaking The whole assay can be completely within 15-30 minutes The detecion of LaRV by one step method is 5-7 days earlier than virus isolations and antibody assays It was specific,sensitive,simple,rapid and reproducible,and can replace convential ELISA and virus isolations It can be used in clinic laboratory and fields
