摘要
利用伪狂犬病病毒特异性扩增产物制成地高辛标记的探针,对闽A株,鄂A株,Bartna株,英国株及临床病料进行检测,其结果与PCR检测结果相符,准确率为100%,灵敏度达到2pgDNA,试验认为该探针具有灵敏、特异。
A digoxin labelled probe prepared form product of amplification of pscudorabies virus was used to detect reference strains of Bartha FA HA Brtain and the clinical samples of piglets infected with pseucorabies virus The results were correspond to that detected by Dolymerase Chain Reaction(PCR) Accuracy rate of nucleic acid probe detection was 100%and the method′s sensibility was 2pg DNA The experiment indicated that the nucleic acid probe was specific, sensitive and safe