hTERT启动子调控的CD基因对卵巢癌细胞株的选择性杀伤作用研究

The selective killing effect of CD gene controlled by hTERT promoter on ovarian cancer cells.

目的探讨由人端粒酶逆转录酶(hTERT)启动子驱动的含胞嘧啶脱氨酶(CD)基因的复制缺陷型腺病毒对卵巢癌细胞的选择性杀伤作用。方法将自行构建的由hTERT启动子调控的携带CD基因的重组腺病毒分别感染人卵巢癌细胞株SKOV3及人胚肺成纤维细胞,通过MTT法检测受染细胞的存活率,观察分析腺病毒载体对细胞的杀伤作用。结果用不同滴度的重组腺病毒以及不同浓度的5-FC作用于SKOV3细胞后,MTT法检测到细胞的存活率随着病毒滴度和5-FC浓度的增加而不断降低;而同样的处理对人胚肺成纤维细胞的也有部分杀伤作用,但远较SKOV3细胞弱,差异有统计学意义(P<0.01)。结论本实验构建的复制缺陷型腺病毒Ad-hTERTp-CD对SKOV3细胞具有靶向杀伤的能力。

Objective To evaluate the selective killing effects of recombinant adenovirus vectors in which the cytosine deaminase（ CD ）gene is under the control of the human telomerase reverse transcriptase （ hTERT）promoter on ovarian cancer cell in vitro. Metheds SKOV3 cells and human embryo lung fibroblast cells（HELF） transfected with recombined adenovirus were exprosed to medium with 5-FC and MTT assays were performed to measure the cell viabihty. Results After transfected by recombinant adenovirus Ad-hTERTp- CD in vltro,and combined with 5-FC,the cell growth of SKOV3 ovarian cancer cell were suppressed compared with HELF cell（ P 〈 0.01 ）. Conclusion Recombinant adenovirus combined with 5-FC results in a significant killing effect on SKOV3 cells in vitro, but not on HELF cells.