伤寒Vi多糖疫苗大规模生产中的工艺改进

Improved Manufacturing Technique of Vi Polysaccharide Typhoid Vaccine (VPTV)

对伤寒Ｖｉ多糖疫苗大规模生产进行了三项工艺改进：１．将液体菌种由静止期接种大罐改为对数生长期接种大罐，大罐培养时间可缩短３－４小时。２．２％（Ｖ／Ｖ）甲醛溶液杀菌时间由５℃６小时改为２０－２５℃２小时，能保证杀菌完全。３．用冷酚精制Ｖｉ多糖由常规法改为乳化法，可减少精制次数及冷酚用量。

Three methods of technological improvements in process used for the large scale VPTV as were described follows:1.The fermentation time is shortened by 3-4hrs,using inoculated seed stock suspension in exponential phase instead of in stationary phase;2.Bacteria killed time is shortened to 2hrs with 2% formaline at 20-25℃ instead of 6hrs at 5℃;3。To purify Vi polysaccharide by cold phenol extraction with the emulsified solution instead of by a conuentional method without emulsification can reduce purification times and amounts of phenol consumption and increase the yield of products.