基于琼脂糖和纳米金的电流型免疫传感器快速检测副溶血性弧菌

Amperometric Immunosensor for Rapid Detection of Vibrio Parahaemolyticus Based on Agarose and Nano-Au

将HRP-anti-VP掺杂于琼脂糖和纳米金中并修饰于丝网印刷电极表面，从而制备副溶血性弧菌免疫电极．采用循环伏安法表征免疫电极和监测酶促反应，根据还原电流的变化来定量测定副溶血性弧菌．在优化的实验条件下，免疫电极线性检测范围为10^5～10^9cfu／mL，检出限为7．4×10^4cfu／mL（S／N=3）．该免疫电极具备较好的特异性、重现性（RSD〈6％）、稳定性（一周后电流响应为初始值的91％）和准确性（与ELISA符合率为97．5％），可初步用于副溶血性弧菌的快速筛检．

A disposable electrochemical immunosensor for detection of Vibrio parahaemolyticus （VP） based on the screen-printed electrode （SPE） coated with agarose/Nano-Au membrane and HRP-anti-VP has been developed. The immunosensor was characterized by cyclic voltammetry （CV）. VP could be quantificationally detected according to the shift of reduction current while CV was used as electrochemical means to detect the products of the enzymatic reaction. Under the optimum experimental conditions, VP was rapidly detectable by sigmoidal curve method and form a linear calibration between 10^5 and 10^9 cfu/mL with an associated detection limit of 7. 4×10^4 cfu/mL（S/N--3）. The immunosensor had acceptable specificity, reproducibility, stability and accuracy, which indicating the immunosensor could satisfy the need of practical sample detection.