甲状腺素及维甲酸对成骨样细胞甲状旁腺素受体的调节作用

The Effect of Thyroid Hormone and Retinoic Acid on Paratkyroid Hormone Receptor in Osteoblast-like ROS 17/2.8 Cells

研究了甲状腺素（T3／T4）及维甲酸（RA）对大鼠成骨样细胞ROS17／2．8细胞林甲状旁腺素（PTH）受体的调节作用．实验结果表明：细胞经T3／T4处理后，可显著增高PTH受体结合率及碱性磷酸酶活性，以及PTH受体mRNA的表达．细胞经RA处理后，则相反地降低PTH受体结合率及碱性磷酸酶活性．

Parathyroid hormone (PTH) is a potent factor for bone resorption and bone cellmetabolism. The effect of thyroid hormone (T3/T4 ) and retinoic acid (RA) on the regulation ofPTH receptor(PTHR)was investigated. In osteoblast-like ROS 17/2. 8 cells, PTHR binding percentage, PTHR gene expression and alkaline phosphatase were up-regulated after treated with T3/T4 (10－10－10－6 mol/L); While it was down-regulated after treated with RA(10－10－10－5 mol/L). It is suggested that the regulation level of the 'cross talk'among T3/T4, RA and PTH is onthe PTH receptor gene expression.