ADPRT抑制剂对不同辐射敏感性人肿瘤细胞株的作用比较

Comparison of Efects of ADPRT Inhihitor on Human Tumor Cell Lines with Different Radiosensitivity

从细胞的克隆形成能力和细胞DNA双链断裂及修复几方面分析了两个人卵巢癌细胞株HOC8和A2780对电离辐射的敏感性并探讨了ADP-核糖基转移酶（ADPR）的特异性抑制剂3-氨基苯甲酰胺（3－AB）对二者的辐射增敏效应，结果表明A2780细胞的辐射敏感性大大高于HOC8细胞，其D0值分别为0．9和2．5Gy；γ射线所致两株细胞的初始DNA双链断裂水平没有显著差异，但A2780细胞对DNA双链断裂的修复能力比HOC8细胞低．3AB能降低受照细胞的克隆形成能力及细胞对双链断裂的修复能力，其中对HOC8细胞的作用更为明显．

Radiosensitivity of two human tumor cell lines, HOC8 and A2780, and radiosensitization effect of 3-aminobenzamide(3-AB), a specific inhibitor of ADP-ribose transferase(ADPRT)were discussed. Results showed that A2780 was more sensitive to ionizing radiation than HOC8,with Do of 0. 9 Gy and 2. 5 Gy, respectively; No significant difference was seen in initial DNAdouble-strand break(DSB) induction between two cell lines, but A2780 had lower DSB repair capacity. 3-AB reduced both the level of cell survival and DNA DSB repair. This phenomenon wasmore evident on HOC8 cells.