无精子症患者与生育男性精浆蛋白质群的比较分析

Comparative Analysis of Proteins in Seminal Plasma of Non-obstructive Azoospermia Patients and Healthy Fertile Males

目的:探讨无精子症患者精浆蛋白质群的改变。方法:11例正常健康自愿者和6例非梗阻性无精子症者精液标本,以99%和45%Percoll分离获取精浆。采用PBS IIC型蛋白质芯片-飞行时间质谱(SELDI-TOF-MS),经CM10芯片捕获蛋白质,并采用TOF-MS对蛋白质进行检测获得各样本的蛋白质指纹图谱,经过归一化处理后进行组间比较。结果:无精子症组与正常组比较有28种蛋白质表达存在差异,差异的蛋白质中有24种含量低于正常组,其中4个M/Z在7 196.058、7 630.573、7 547.610和7 709.833的蛋白质差异极显著(P<0.01)。结论:无精子症患者其精浆蛋白质群较生育男性有显著改变,改变的蛋白质主要是含量减少,这些蛋白质的减少可能与无精子症的发生有重要相关性。

Objective： To analyse protein alterations in the seminal plasma of non-obstructive azoospermia patients. Methods： men samples were collected from 11 healthy fertile and 6 azoospermia male volunteers respectively and tested by SELDI-TOF-MS with CM10 protein chip to get protein spectra maps, which were automatically treated with the special soflwares of Ciphergen Inc. Results： The mean peak heights of 28 proteins expressed in the seminal plasma of the azoospermia patients were statistically different from those of the healthy fertile males （P 〈 0.05 ） , of which 24 were of lower contents than in the normal controls, 4 with remarkably significant difference, M/Z7 196.058, 7 630.573, 7 547.610 and 7 709.833（P 〈0.01）. Conclusion： The seminal plasma proteins of the azoospermia patients were significantly different from those of the healthy fertile males, with decreased contents of most of the different proteins, which might be significantly correlated with the development of azoospermia. Natl J Androl,2007,13 （7） ：579-583