白藜芦醇对2,5-己二酮导致SD大鼠睾丸生精障碍治疗作用的实验研究

Resveratrol Helps Restore Spermatogenesis After Testis Injury Induced by 2,5-hexanedione

目的:观察白藜芦醇促进2,5-己二酮(2,5-HD)所致睾丸生精障碍后的生精功能恢复作用。方法:40只雄性SD大鼠随机分为5组(每组8只),A组正常喂饲,B、C、D、E组均饮用含1%2,5-HD的水溶液5周,然后C、D、E组用不同浓度的白藜芦醇[分别为20、40、80 mg/(kg.d)]治疗9周。比较各组外表体征、体重增加值、睾丸重量的变化,及各组生精小管数目、直径以及生精细胞膜c-kit蛋白表达水平。结果:与A组相比,B、C、D、E组大鼠体质瘦弱、皮肤松弛、毛色暗淡、体重增长减慢、睾丸萎缩;睾丸组织HE染色及免疫组化染色显示生精上皮萎缩,生精细胞发育停滞,c-kit蛋白表达受到抑制。经过白藜芦醇治疗后,C、D、E组大鼠外表体征得到恢复,接近A组,体重和睾丸重量均较B组有所恢复(P<0.01);睾丸生精功能部分恢复,但生精小管数目、直径低于A组水平(P<0.001),同时c-kit蛋白重新获得表达,但低于A组水平(P<0.01)。随着白藜芦醇用量增加,C、D、E组大鼠睾丸生精小管数目、直径显著恢复(P<0.01),生精细胞膜c-kit蛋白表达量增加更为明显(P<0.01)。结论:白藜芦醇可以促进2,5-HD所致大鼠睾丸生精障碍的生精功能恢复。

Objective： To study the effect of resveratrol on spermatogenesis after 2,5-hexanedione （2,5-HD）-induced testicular injury. Methods： Forty male SD rats were randomly divided into 5 groups. Group A were normally raised and Group B, C, D and E exposed to 1% 2,5-HD for 5 weeks, followed by administration of resveratrol of different concentrations （20, 40 and 80 mg/[kg · d], respectively） to Group C, D and E for 9 weeks. Then the rats were killed, their physical signs, body weight gain and testis weight were assessed, and immunohistochemistry and Western blot analysis used to investigate the numbers and diameters of seminiferous tubules and the expression of c-kit protein of spermatogenic cell membrane. Results： The rats exposed to 2,5-HD showed weak body, lax skin, dim color pattern, tardy body weight gain, and emaciated testis. Immunohistochemistry revealed emaciated seminiferous tubules, stagnant obsolete spermatogonia and negative expression of c-kit protein. After resveratrol administration, the 2,5-HD-induced physical signs were improved and close to normal. Compared with those of the 2,5-HD injured group, the body weight and testis weight of the resveratrol treated group increased obviously （P 〈 0.01 ） ; and the aliquots of the seminiferous epithelia began to differentiate and thespermatogenesis and expression of c-kit protein partly resumed （P 〈 0.01 ）. With increasing dose of resveratrol, the diameters and numbers of seminiferous tubules （ P 〈 0.01 ） and the expression levels of c-kit protein （ P 〈 0.01 ） were gradually and significantly restored almost to normal. Conclusion ： Resveratrol could promote the recovery of spermatogenesis after 2,5-HD-induced testicular injury.