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PLAGL2与SP-C基因启动子相互作用的研究 被引量：4

Interaction between PLAGL2 and SP-C Gene Promoter
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摘要 目的研究PLAGL2(pleiomorphic adenoma gene like-2,PLAGL2)对SP-C基因(surfactant pro-tein-C,SP-C)启动子的结合与影响作用。方法定点诱变技术(site-directed mutagenesis)及凝胶电泳迁移实验(electrophoresis mobility shift assay,EMSA)研究PLAGL2与SP-C基因启动子之间的相互结合作用;体外培养MLE12细胞共转染和荧光素酶报告基因活性值检测技术,研究PLAGL2对SP-C基因启动子表达活性的影响;结果EMSA及竞争性EMSA实验表明:PLAGL2能通过其核心和簇结合位点序列与同位素标记的SP-C基因启动子片段相结合形成蛋白-DNA复合物;细胞共转染及荧光素酶报告基因活性值检测结果显示:PLAGL2能明显促进SP-C基因启动子的表达。结论在肺Ⅱ型细胞中,PLAGL2能与SP-C基因启动子相互作用并促进其表达。 Objective To Study the interaction between PLAGL2 and SP-C gene promoter. Methods Site-directed mutagenesis and EMSA were conducted to study the ability and specificity of interaction between PLAGL2 and SP-C gene promoter DNA fragment ; Co-transfaction and luciferase activity measurement were employed to examine the effect of PLAGL2 on sp-c gene expression Results EMSA and competitive EMSA showed that in vitro translated PLAGL2 protein could bind to SP-C gene promoter probe to form the protein-DNA retarded complex by it＇s core and cluster binding sequence. Luciferase activity measurement revealed that PLAGL2 substantially transactivated the SP-C gene promoter in co-transfaction （P 〈0. 001 ） . Conclusion PLAGL2 could greatly promote the SP-C gene promoter activity in the lung cells.
作者 邓飞涛 王泽华 夏炎枝 吴超英 柴新群
机构地区 华中科技大学附属协和医院妇产科 华中科技大学同济医学院细胞生物学系 华中科技大学附属协和医院肝胆外科
出处 《医学分子生物学杂志》 CAS CSCD 2007年第4期289-292,298,共5页 Journal of Medical Molecular Biology
关键词 PLAGL2蛋白 SP—C启动子 荧光素酶报告基因检测 凝胶电泳迁移实验 PLAGL2 SP-C promoter luciferase activity measurement EMSA
分类号 Q344.14 [生物学—遗传学]
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参考文献10

  • 1LANDRETTE S F,KUO Y H,,HENSEN K,et al.PLAG1and PLAGL2are oncogenes that induce acute myeloid leukemia in co-operation with Cbfb-MYH11[].Blood.2005
  • 2VOZ ML,AGTENNS,WINJ M,et al.PLAG1,the main trans-location target in pleomorphic adenoma of the salivary gland,Is a Positive regulatior of IGF-II[].Cancer Research.2000
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  • 7Kas K,Voz ML,Roijer E,et al.Promoter swapping between the genes for a novel zinc finger protein and beta-catenin in pleiomorphic adenomas with t(3;8)( p21;q12) translocations[].Nature Genetics.1997
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  • 10Meng-Chun Yang Yuhong Guo Chia-Chi Liu,Jonathan C. Weissler and Yih-Sheng Yang.The TTF-1/TAP26 complex differentially modulates surfactant protein-B (SP-B) and -C (SP-C) promoters in lung cells[].Biochemical and Biophysical Research Communications.2006

同被引文献19

  • 1Weaver TE, Conkright JJ. Function of surfactant proteins B and C [ J]. Annu Rev Physiol, 2001, 63:555.
  • 2Nogee LM. Abnormal expression of surfactant protein C and lung disease [J] . Am J Respir Cell Mol Biol, 2002, 26:641.
  • 3Jacot W. Bousquet J. Understanding the regulation of surfactant gene expression [J] . Eur Respi J, 2003, 22:6.
  • 4Bachurski CJ, Kelly SE, Glasser SW et al. Nuclear factor Ⅰ family members regulate the transcription of surfactant protein -C [J] . J Biol Chem, 1997, 272:32759.
  • 5Glasser SW, Burhans MS, Eszterhas SK et al. Human SP - C gene sequence that confer lung epithelium - specific expression in transgenic mice [J] . Am J Physiol Lung Cell Mol Physiol, 2000, 278:L933.
  • 6Liu C, Glasser SW, Wan H et al. GATA -6 and thyroid transcription factor - 1 directly interact and regulate surfactant protein - C gene expression [J] . J Biol Chem, 2002, 277:4519.
  • 7Park KS, Whitsett JA, Di Palma T et al. TAZ interacts with TTF -1 and regulates expression of surfactant protein - C [ J] . J Biol Chem, 2004, 279:17384.
  • 8Yang MC, Gu Y, Liu CC et al. The TTF - 1/TAP26 complex differenti-ally modulates surfactant protein - B ( SP - B) and - C ( SP - C) promoters in lung cells [ J] . Biochem Biophys Res Commun, 2006, 344 (2): 484.
  • 9Abdollahi A. LOT1 (ZAC1/PLAGL1) and its family members: mechanisms and functions [ J] . Journal of Cellular Physiology, 2007, 210:16.
  • 10Kas K, Voz ML, Hensen K et al. Transcriptional activation capacity of the novel PLAG family of zinc finger proteins [ J ] . J Biol chem, 1998, 273:23026.

引证文献4

二级引证文献5

医学分子生物学杂志
2007年 第4期

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