葛根素对大鼠脑缺血再灌注损伤的保护作用

Protective effect of puerarin agaist cerebral ischemia-reperfusion injury in rats

目的观察葛根素对大鼠脑缺血再灌注损伤的保护作用。方法采用线栓法复制大鼠大脑中动脉脑缺血再灌注损伤模型,检测血液流变学指标,并测定再灌注后缺血侧脑组织含水量、丙二醛(MDA)含量、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性。结果葛根素0.5、0.25、0.125 g.kg-1均可不同程度降低高、中、低切变率全血黏度、红细胞压积和血沉,降低缺血脑组织含水量和MDA含量,提高脑组织T-AOC,升高SOD活性。结论葛根素对大鼠大脑中动脉缺血再灌注损伤具有明显的保护作用。

Aim To study the protective effect of puerarin（Pue） against cerebral ischema-reperfusion injury in rats.Methods SD rats were treated with Pue for 6 days and were prepared to focal ischemia with middle cerebral artery occlusion（MCAO）.After 2 hours,perfusion was recovered by pulling out the suture.12 hours later,hemorrheology indexes were measured and the homogenate of brain tissue were obtained after reperfusion.The contents of water and malondialdehyde（MDA） levels,total antioxidant capacity（T-AOC） and the activities of superoxide dismutase（SOD） and（T-AOC） were determined by spectroscopy.Results Hemorheological research showed that Pue reduced whole blood viscosity,hematocrit（HCT）,erythrocyte sedimentation rate（ESR）.Pue decreased the contents of water and MDA,elevated the activities of SOD and T-AOC.Conclusion It is suggested that Pue can improve hemorheological state and protect against cerebral ischemia-reperfusion injury by enhancing the activity of anti-oxidative enzymes,inhibiting lipid peroxidation subsequently and attenuating free radicals-mediated brain damage.