摘要
选用前人分离得到的42对大熊猫微卫星引物,分别用圈养大熊猫的血液DNA和野生大熊猫的粪便DNA对其进行PCR扩增,并比较琼脂糖凝胶电泳和变性聚丙烯酰胺凝胶电泳的检测结果。结果表明,不同标记的多态性差异较大,筛选出13对能较好地应用于大熊猫遗传多样性研究的微卫星引物。
A total of 42 microsatellites of the giant panda （Ailuropoda melanoleuca） were evaluated through PCR amplification. The results showed that great variations on the polymorphism were observed among these markers and 13 microsatellites with high polymorphism were selected.
出处
《四川动物》
CSCD
北大核心
2007年第3期531-533,共3页
Sichuan Journal of Zoology
关键词
微卫星
大熊猫
遗传多样性
microsatellite
giant panda
genetic diversity
