正交试验法优选养血扶正颗粒醇提部分的工艺研究

Study on Technique of Ethanol-extraction Part of Yangxuefuzheng Granules by Orthogonal Test

目的:正交试验法优选养血扶正颗粒的乙醇提取工艺。方法:以浸膏得率及淫羊藿苷的含量作为考核指标,通过L9 (34)正交试验设计比较醇提与醇提水沉的提取工艺条件,确定养血扶正颗粒的最佳醇提工艺。结果:醇提水沉提取工艺影响因素大小依次是:浸渍时间(C)>乙醇浓度(D)>加醇量(A)>浸渍次数(B),最佳提取工艺为处方药材加10倍量乙醇,浸渍提取1次,每次5 d,乙醇浓度65%。结论:醇提水沉淀后浸膏得率及淫羊藿苷的含量有所降低,醇提法优于醇提水沉法,该提取方法合理、可行,为养血扶正颗粒醇提工艺提供了实验依据。

Objective： The orthogonal test was used to optimal ethanol-extraction technique of Yangxuefuzheng granules. Method： The extraction yield and the content of ieariin were used as index, the optimum extraction conditions of Yangxuefuzheng granules were studied with the orthogonal test of L9 （ 34 ）, the effect of ethanol extraction and ethanol extracting-water precipitating were compared. Result： For ethanol extraction technique, the influencing power of factor was described as： C 〉 A 〉 B 〉 D, the optimum preparation conditions were A3 B1 C1 D1 ; For ethanol extracting-water precipitating technique, the effect order of factors was C 〉 D 〉 A 〉 B, the optimum preparation conditions were A3 B1 C1D1 （ A： added ethanol volume, B： immersion times, C ： immersion frequency, D： the concentration of ethanol）, i.e. added 10 times ethanol, immersion for 1 times, 5 day every time and added 65% ethanol. Conclusion： After ethanol extracting-water precipitating, the extraction yield and the content of ieariin are decreased. Ethanol extraction technique is better than ethanol extracting-water precipitating technique. The optimum extraction technique is rational, feasible, and the experiment results provide the basis for the extraction technique of Yangxuefuzheng granules.