HPLC-DAD、HPLC-MS联用检测中药中混入莨菪碱类成分

Detection the Mixture of Hypscyamine Composition into Traditional Chinese Medicine by HPLC-DAD Combined with HPLC-MS

目的:建立一种简便、快速、灵敏、准确地鉴定中药中莨菪碱类成分混入的高效液相色谱-阵列二极管检测器(HPLC- DAD)、高效液相色谱-串联质谱(HPLC-MS)分析方法。方法:色谱柱为Lichrospher Si正相色谱柱(250 mm×4.6 mm,5μm), DAD检测条件:流动相:30 mmol·L-1醋酸铵溶液-乙腈=30:70,检测波长210 nm,流速1.0 ml·min-1。HPLC-MS以离子阱为检测器,采用正离子检测模式进行检测。结果:运用此方法成功的确证中药煎剂中阿托品、山莨菪碱、东莨菪碱类成分。结论:本方法简便准确,可做为中药中莨菪碱类成分混入的检测方法。

Objective： To establish a way to identify the mixture of hypscyamine component into TCM by HPLC-DAD combined with HPLC-MS. Method： Lichrospher Si normal phase chromatographic column （250 mm × 4.6 mm, 5 μm） was used and DAD was taken as the detection; 30 mmol·L^-1 ammonium acetate solution methanol and acetonitrile（30： 70） were used as mobile phase,detection wavelength was at 210 nm and flow rate was 1.0 ml·min^-1. Ion trap was used as the detection in HPLC-MS with the positive ion model. Result： Atropine, Anisodamine and scopolamine were identified successfully in the TCM decoction. Conclusion： The method is easy and reliable, which can be used to detect the mixture of the hypscyamine into the TCM.