摘要
目的:观察扶正利湿解毒法基本方(简称基本方)对肝纤维化的逆转作用.方法:采用DMN诱导大鼠肝纤维化,造模结束后随机分为模型对照组,秋水仙碱组,基本方高剂量组,基本方中剂量组及基本方低剂量组并分组给药,同时设空白对照组.灌胃3wk后检测肝组织Hyp,ALT水平;HE,Masson染色.采用HSC-T6细胞培养,药物作用24h后光镜下观察拍照,检测药物对细胞的抑制率并在电镜下观察药物浓度为2g/L时细胞的变化.结果:基本方高、中剂量均可使大鼠肝组织Hyp,血清ALT显著下降(Hyp:213.73±58.05μg/g,245.95±67.70μg/g vs 317.37±42.06μg/g,P<0.01,P<0.05;ALT:33.56±8.16 kU/L,42.38±14.99 kU/L vs 58±10.32 kU/L,P<0.01,P<0.05);Masson染色肝纤维化分级评分显示,基本方高,中剂量均可使肝纤维化程度显著降低(2.33±0.87,2.63±0.74 vs 3.70±0.67,P<0.01,P<0.01),同时显著改善肝组织炎症、出血及纤维沉积;光镜观察及抑制率检测显示基本方呈剂量依赖性抑制HSC-T6细胞增值.电镜显示基本方主要导致HSC-T6细胞凋亡.结论:基本方显示了显著的体内外逆转肝纤维化作用.
AIM: To observe the reversing effect on liver fibrosis by the therapy of strengthening healthy qi combined with draining dampness and relieving toxin (SHQDDRT).
METHODS: Rat model of liver fibrosis was induced by dimethylnitrosamine (DMN). All the rats were randomly divided into 6 groups, named group A (control, n = 10), B (model, n = 10), C (colchicine, n = 10), D (with the basic herb complex, high dose, n =10), E (with the basic herb complex, middle dose, n =10) and F (with the basic herb complex, low dose, n = 10). The content of liver hydroxyproline and the serum aμnine aminotransferase (ALT) were tested. HE and Masson staining were conducted for patho-logical examination. HSC-T6 cell line was used as an in vitro model. After 24 hours of drug administration, the inhibitory effect was observed under an optical microscope. Cell prolifera- tion was detected by MTT assay. Additionally, HSC-T6 cells were observed by electron microscopy after they were treated with the basic herb complex and control drug at the concentration of 2 g/L.
RESULTS: The content of liver hydroxyproline and the serum ALT in group D and E were significantly lower than those in group B (hydroxyproline: 213.73 ±58.05 μg/g, 245.95 ±67.70 μg/g vs 317.37±42.06 μg/g, P 〈 0.01, P 〈 0.05; ALT: 33.56± 8.16 kU/L, 42.38±14.99 kU/L vs 58±10.32 kU/L, P 〈 0.01, P 〈 0.05). Masson staining showed that the fibrotic degrees were significantly decreased in group D and E in contrast with those in group B (2.33 ± 0.87, 2.63 ± 0.74 vs 3.70± 0.67, both P 〈 0.01), and the fiber deposition, infμmmation and hemorrhage were also improved. Light endoscopy and MTT assay demonstrated that the basic herb complex inhibited the growth of HSC-T6 cells in a dose- dependent manner. Electron microscopy exhibited typical apoptosis features in HSC-T6 cells.
CONCLUSION: The basic herb complex shows significant reversing effect on liver fibrosis in vitroand in vivo.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第16期1781-1787,共7页
World Chinese Journal of Digestology
基金
广州中医药大学科研创新基金
No.k0050044~~
关键词
肝纤维化
逆转作用
复方
MASSON染色
细胞凋亡
Liver fibrosis
Reversing effect
The basic herb complex
Cell apoptosis
Masson staining