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热休克蛋白10、27和47在多囊卵巢综合征患者卵巢中的表达 被引量：5

Expression of HSP10, HSP27 and HSP47 in Polycystic Ovary Syndrome
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摘要 目的:探索热休克蛋白(heat shock protein,HSP)10、27和47在多囊卵巢综合征(PCOS)患者卵巢组织中的表达及其意义。方法:采用荧光实时定量PCR(SYBR GREEN Iassay)、Western blot和免疫组织化学技术分别对3例正常妇女(对照组)、3例无排卵PCOS患者(PCOS组)卵巢组织的HSP10、HSP27和HSP47的mRNA及蛋白进行测定。结果:①HSP10 mRNA在PCOS患者卵巢组织的表达与对照组相比,差异无显著性(P>0.05);HSP10蛋白在PCOS患者卵巢组织的表达与对照组相比,表达降低,差异有显著性(P<0.05);免疫组化结果显示HSP10表达于对照组卵巢的所有细胞,而在PCOS组仅在卵泡膜细胞表达水平较高。②HSP27 mRNA和蛋白在PCOS患者卵巢组织的表达降低与对照组相比,差异有显著性(P<0.05);免疫组化结果显示HSP27高表达于对照组卵巢原始卵泡的卵母细胞。③HSP47 mRNA和蛋白在PCOS患者卵巢组织的表达高于对照组,PCOS组卵巢,差异有显著性(P<0.05);免疫组化结果显示HSP47高表达于PCOS组卵巢的卵母细胞、颗粒细胞和间质细胞。结论:HSP10、HSP27和HSP47在PCOS患者卵巢和正常人卵巢中存在差异表达。 Objective： To explore the expression of HSP10, HSP27 and HSP47 in polycystic ovary syndrome. Methods： Real-time PCR,Western blot analysis, immunohistochemistry were carried out to define the experssion of liSP10, HSP27 and HSP47 in 3 PCOS ovaries and 3 normal ovaries. Results： 1） HSP10 mRNA didn＇t have significant differential expression between PCOS and normal ovaries （P〉0.05）; HSP10 protein had a high expression in normal ovaries （P〈0.05）, and it was highly expressed in all cells of normal ovaries, but only highly expressed in theca cells of PCOS ovaries. 2） Both of HSP27 mRNA and protein were highly expressed in normal ovaries （P〈0.05）; HSP27 was highly expressed in oocyte of normal ovaries. 3） Both of HSP47 mRNA and protein were highly expressed in PCOS ovaries （P〈0.05）; HSP47 was highly expressed in ooeytes, granulosa cells and stroma cells of PCOS ovaries. Conclusion： All of HSP10, HSP27 and HSP47 have significant differential expression between PCOS and normal ovaries.
作者 樊璐 刘嘉茵 马翔
机构地区 南京医科大学第一附属医院临床生殖中心
出处 《生殖与避孕》 CAS CSCD 北大核心 2007年第6期393-398,409,共7页 Reproduction and Contraception
关键词 多囊卵巢综合征(PCOS) 卵巢 热休克蛋白10 热休克蛋白27 热休克蛋白47 polysystic ovary syndrome （PCOS） ovary HSP10 HSP27 HSP47
分类号 R711.75 [医药卫生—妇产科学]
  • 相关文献

参考文献18

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二级参考文献33

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共引文献9

同被引文献48

  • 1沙红英,陈建泉,钱旻,成国祥.哺乳动物卵泡发育的调控机制[J].生殖与避孕,2004,24(5):295-298. 被引量：10
  • 2成毅明,石心泉,于和鸣,吴燕婉,贾孟春.大鼠精子发生过程中热休克蛋白70-2特异表达的研究[J].生殖医学杂志,2005,14(6):346-351. 被引量：3
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引证文献5

二级引证文献15

生殖与避孕
2007年 第6期

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