蜡蚧轮枝菌昆明菌株发酵培养技术研究

Study on Fermentation and Culture Technology of Verticillium lecanii

用5种培养液对蜡蚧轮枝菌昆明菌株（KM9803）进行液体培养的结果：以改良的沙氏培养液和豆粉-土豆汁培养液中芽生孢子产量较高；摇瓶时间以4—5d较适宜。产孢量可以达到7．26×10^8-9．09×10^8个芽生孢子／mL；固体培养阶段培养基：加水量以1：0．6为宜，固体培养发酵时间以7d为宜，分生孢子产量可达到4．05×10^9-4．11×10^9孢子／g。

Blastospore yields of KM9803 in 5 kinds of liquid mediums shows that high blastospore yields can be achieved in SDAY and potato liquid, it＇ s about 7.26 × 10^8 - 9. 09 × 10^8 blastspore/mL and just about 4 -5 days for fermentation; Medium： water is 1.0 to 0. 6. The conidiophore yields of KM9803 in solid medium can reach to 4. 05 × 10^9 -4. 11 × 10^9 conidiophore/g and need 7d for fermentation.