ACE基因多态性与新诊断2型糖尿病患者颈总动脉内中膜增厚的关系研究

Association of ACE gene polymorphisms with the carotid intima-media thickness in type 2 diabetes

目的探讨人血管紧张素转换酶(ACE)基因插入/缺失多态性(I/D)与新诊断2型糖尿病(T2DM)患者颈总动脉内中膜厚度(CCA-IMT)的关系。方法采用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)技术,检测2005年5月至2006年5月东莞市人民医院内分泌门诊收治的120例T2DM患者ACE基因内含子16I/D多态性,利用B超检测其CCA-IMT,通过多元Logistic回归分析筛选T2DM患者CCA-IMT增厚的危险因素。结果携带ACE基因DD型者CCA-IMT增厚的比例显著高于携带II及ID基因型者(F=10.164,P=0.006);Logistic回归分析显示,ACEDD基因型、年龄、合并高血压是T2DM患者CCA-IMT增厚的危险因素(OR分别为3.689,1.054,1.562,P均<0.05)。结论ACEDD基因型是T2DM患者CCA-IMT增厚的独立危险因素。

Objective To investigate the relationship between angiotensin l-converting enzyme（ACE）inserting/defaulting（I/D ）gene polymorphisms and the carotid intima-media thickness （CCA-IMT）in patients with type 2 diabetes （T2DM）. Methods The polymorphisms of ACE（I/D） was determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method and the CCA-IMT was assessed using non-invasive high resolution B-mode ul- trasonography in 120 T2DM in Han＇s population of Dongguan city. The data were analyzed by Logistic regression to explore the independent and significant risk factors for CCA-IMT increase in type 2 diabetic patients. Results The DD genotype were significantly more common in diabetic patients with CCA-IMT increase than in those without it（ F = 10. 164,P = 0. 006） ; Logistic regression analysis showed that DD genotype, age, male gender and essential hypertension contributed significantly to the CCA-IMT increase in type 2 diabetic patients （ OR = 3. 689,1. 054,1. 562, respectively, P 〈 0.05 ）. Conclusion DD genotype is an independent risk factor for CCA-IMT increase in type 2 diabetic patients.