新麦草遗传多样性等位酶分析

Allozyme genetic diversity analysis of Psathyrostachys juncea

采用不连续系统的聚丙烯酰胺垂直板凝胶电泳(VPAGE)对新疆新麦草的5个天然居群和1个栽培种遗传多样性和居群结构进行了分析。测定的6个酶系统中,确定了9个等位酶位点,多态位点百分率平均值为90.48%,平均等位基因数为2.920 7,平均预期杂合值为0.542 7。总的基因多样性中,86%存在于居群内,14%来自居群间。聚类分析以遗传距离D=0.14为分界线将6份材料明显地分为2组。研究表明,新麦草具有丰富的遗传多样性,其多样性可能与生境、人工驯化、风媒异交等因素有关。

Allozyme genetic diversity of 5 natural populations and 1 cultivar of Psathyrostachys juncea originated from Xingjian, China were analyzed by vertical polyacrylamide gel electrophoresis. Six enzymes revealed 9 loci. The mean of percentage of polymorphic loci （P） was 90.48%o and number of alleles per locus （Ae） was 2. 920 7. The mean of the expected heterozygosity （He） was 0. 5427. Eighty six percent of the diversity resided within populations （GST = 0. 14）. The cluster analysis indicated that 6 accessions were separated into two distinct groups from genetic D=0.14. And the result indicated that 6 populations had high genetic diversity and may attribute to the environment, domestication and cross-pollination.