复方苦参注射液对胃癌SGC-7901细胞侵袭转移能力的影响

Effect of Matrine Injections on Invasion and Metastasis of Gastric Carcinoma SGC-7901 Cells in Vitro

目的:研究复方苦参注射液对胃癌SGC-7901细胞侵袭转移能力的影响。方法:体外培养胃癌细胞系SGC-7901,MTT法测定药物对细胞增殖的抑制作用,应用粘附试验、Transwell趋化运动和Matrigel侵袭实验,研究复方苦参注射液及其不同浓度下对SGC-7901细胞的粘附、运动以及侵袭能力的影响。结果:复方苦参注射液浓度在0.25、0.5、1.0、1.5 mg/ml时均有一定的抑制胃癌SGC-7901细胞增殖的作用,且呈明显的量效和时效关系。复方苦参注射液不同浓度处理SGC-7901细胞后,体外粘附、侵袭和运动能力呈剂量依赖性下降。经复方苦参注射液(1.5 mg/ml)作用后SGC-7901细胞CD44V6蛋白表达减弱,胞浆棕黄色颗粒减少。结论:复方苦参注射液可以抑制胃癌细胞生长,降低细胞粘附、侵袭和运动能力,其机制可能与抑制CD44V6蛋白表达有关。

Objective： To investigate the effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro. Methods ： MTT assay was used to examine the effect of matrine injections on proliferation of SGC-7901 cells after 24, 48, 72, 96 hours treatment ; Transwell chamber assay was perfotraed to determine the effect of matrine injection on invasion and migratory capacity of the cells; Effect on adhesion potential of SGC-7901 cells was tested by cell matrigel adhesion assay. Results： Matrine injections could inhibit the proliferation of SGC-7901 cells, with obvious dose-dependent and time-dependent effects. Matrine injections significantly inhibited adhesion, invasion and migration capacity of SGC-7901 cells in vitro. The inhibitory rate of them after treatment with 1.5 mg/ml matrine injections for 24 h were （45.32 ± 3. 10 ） %, （ 32. 66 ± 2. 82 ） % , （ 38. 35 ± 3.41 ） % respectively. After treat- ment of matrine injections（ 1.5 mg/ml）for 24 h, the expression level of CD44v6 in SGC-7901 cells was decreased compared with the untreated group. Conclusion： Matrine injections can inhibit the migration, invasion and adhesion capacity of SGC-7901 cells in vitro. The inhibition effect may be related to down-regulating the expression of CD44v6 protein.