楮实子生物碱的细胞毒作用研究

Cytoxic Activities of Total Alkaloids Isolated from Fructus Broussonetiae in Vitro

目的:对楮实子总生物碱抗肿瘤细胞的活性进行探讨。方法:分别用MTT及集落形成法考察楮实子中提得的总生物碱对Hela、BEL-7402、A375、SMM1990、Saos-2细胞株的细胞毒作用。结果:当药物浓度在10μg/L以下,抑制作用不明显。随着药物浓度的升至50μg/L时,药物对肿瘤细胞生长的抑制作用较明显,MTT法测得抑制率在20%以上,集落形成法测得抑制率在30%以上。而当药物浓度达到100μg/L时,楮实子总生物碱显示出较为显著的肿瘤细胞抑制作用,两种方法的细胞生长抑制率分别在50%和60%以上。结论:楮实子中总生物碱有较强的细胞毒作用。

Objective ： To observe the cytoxic activities of total alkaloids isolated from Fructus Broussonetiae on the growth inhibition in five carcinoma cell lines. Methods ： The growth inhibition was analyzed by MTT, Cell colony in the Hela, BEL-7402, A375, SMM1990,Saos-2 cell lines. Results： The growth of all tumor ceils were inhibited by total alkaloids isolated from Fructus Broussonetiae. Conclusion ： The results show that total alkaloids isolated from Fructus Broussonetiae possess the role of antitumors in cell culture.