摘要
本文以山药中总皂甙提取率为考察指标,应用单因素试验和正交设计对山药总皂甙提取工艺中的浸提温度、乙醇的体积分数、浸提时间、料液比进行了研究。以薯蓣皂甙为标准,香草醛-高氯酸为显色剂,冰醋酸为溶剂,用分光光度计在波长为544 nm处对山药总皂甙的含量进行了测定。得出山药总皂甙的最佳提取工艺条件是:浸提温度为60℃,浸提时间为6 h,乙醇体积分数为80%,料液比为1∶8。
A method for the determination of total saponins of Chinese yam was estblished. The dioscin was used as a standard compound, the vanillin-perehloric acid as chromogenic agent and glacial acetic acid as solvent. The extraction technique of asponins from Chinese yam was studied by spectrometric method. Extracting temperature, extracting time, ethanol concentration and the ratio of raw material and water were selected as four factors to design the orthogonal test, and the optical condition of extraction was obtained. The results showed that the optical condition of extraction was as following： extracting temperature 60℃, extracting time 6h, ethanol concetration 80%, and the ratio of raw material and water 1： 8.
出处
《中药材》
CAS
CSCD
北大核心
2007年第7期854-857,共4页
Journal of Chinese Medicinal Materials
关键词
山药
皂甙
提取
分光光度法
Chinese yam
Saponins
Extraction
Spectrometric method
